Performance of the Focus and Kalon enzyme-linked immunosorbent assays for antibodies to herpes simplex virus type 2 glycoprotein G in culture-documented cases of genital herpes. Detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in African sera by using recombinant gG2, Western blotting, and gG2 inhibition. Herpes simplex virus type 2 (HSV-2) IgG index values in two immunoassays in relation to HSV-2 IgG inhibition assay results. Inhibition values >60% indicate true HSV-2-specific IgG reactivity Index value for serum pre-incubated with HSV-1 lysate = 2.45 Index value for serum pre-incubated with HSV-2 lysate = 0.23 An example of how the inhibition value is calculated is shown below: The HSV-1 lysate controls for any non-gG2 reactivity present in the serum sample thus, any inhibition that is detected reflects only the absorption of gG2 reactive antibodies by the native gG2 present in the HSV-2 lysate. The index value of serum pre-incubated with HSV-2 lysate is then compared to the index value of serum pre-incubated with HSV-1 lysate. 2 Separate aliquots of the patient sample are incubated with the 2 lysates, then tested in the HerpeSelect HSV-2 type-specific IgG ELISA. The HSV-2 IgG inhibition assay measures the differential abilities of lysates of cells infected with HSV-1 or HSV-2 to neutralize patient sample reactivity to recombinant gG2 protein (rgG2), the HSV-2–specific protein used in the HerpeSelect HSV-2 IgG ELISA.
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